ABSTRACT
AIM:To explore the relationship between the different stages of diabetic retinopathy and the related factors of vascular endothelial function,and to provide a theoretical basis for improving the function of vascular endothelium to find a way to delay or even inhibit the occurrence or progression of DR.METHODS:We collected during March 2015 to December 2015 in Department of Ophthalmology and endocrinology in our hospital,178 cases of type 2 diabetes mellitus patients and 62 cases of blood specimen in health control group.According to the results of fundus fluorescence angiography (FFA),they were divided into four groups,diabetes patients without retinopathy,diabetes patients with non proliferative diabetic retinopathy (NPDR),diabetes patients with proliferative diabetic retinopathy (PDR) and healthy control group.We detected blood samples of antithrombin Ⅲ (AT-Ⅲ),fibrinolytic enzyme activation inhibitor (PAI),the original organization type fibrinolytic enzyme activator (t-PA) index and the correlation of diabetic retinopathy in installment.RESULTS:This study showed that AT-Ⅲ was significantly different among the four groups (F=5.986,P＜ 0.01);PAI was significantly different among the patients without DR,patients with NPDR and patients with PDR (F=7.434,P＜0.01);t-PA was not significantly different among the four groups (F=2.556,P＞ 0.05);there were relations between the different stages of diabetic retinopathy and AT-Ⅲ,PAI.CONCLUSION:The degree of diabetic retinopathy has a close relationship with the content of antithrombin Ⅲ and plasminogen activator inhibitor,and it is closely related to the function of vascular endothelium.
ABSTRACT
This study was aimed to investigate the effects of bortezomib combined with 5-azacytidine on the apoptosis of K562 cells and expressiom of SHIP mRNA. The K562 cells were cultured and treated with different concentrations of bortezomib, 5-azacytidine or their combination for 24 hours. Then, the expression of SHIP mRNA was detected by RT-PCR,the cell proliferation was analyzed by using MTT assay and flow cytometry. The results showed that 5-20 nmol/L bortezomib could effectively inhibit the proliferation of K562 and this inhibitory effect gradually enhanced along with the increase of bortezomib concentration, the group of bortezomib combined with 5-azacytidine showed more inhibitory effect on K562 cells than that of bortezomib or 5-azacytidine alone.The bortezomib could promote the apoptosis of K562 cells in a dose-dependent manner,and this apoptotic effect was higher in group of bortezomib combined with 5-azacytidine than that in group of bortezomib or 5-azacytidine alone.Bortezomib could down-regulated the expression of SHIP mRNA in a dose-dependent manner,and this down-requlated effect was higher in group of bortezomib combined with 5-azacytidine than that in group of bortezomib or 5-azacytidine alone.It is concluded that bortezomib and 5-azacytidine can induce apoptosis by inhibiting the expression of SHIP mRNA in K562 cells.The combination of bortezomib with 5-azacytidine displays a synergetic effect.